Warriors of Rock is the sixth main game in the rhythm game series. Similar to the previous games it focuses on the band experience, allowing up to four people to sing vocals, or play lead and bass/rhythm guitar, and drums. For the game's music selection this version returns to the series' roots with a less wide spectrum of genres and more focus on rock 'n roll songs.

The game's main career mode is called the Quest mode. It has players go through a campaign here they must defeat a mechanized creature called the Beast by helping save the Demigod of Rock. Players recruit characters by completing some songs in their genre. When successful, the character transforms into a warrior, receives a unique power, and is added to the band's roster. Other game modes are Quickplay+ and Party mode. In the latter one or multiple players go through different songs like in a jukebox, with the ability to drop in or out at any time. The Wii version adds DS functionality with the Roadie mode as introduced in the previous version of the game. It has been expanded to three different game modes and supports up to eight players simultaneously. New abilities for the roadies are now for instance to throw powers at the different players (as unlocked in the Quest mode) to help or sabotage them.

All previously bought tracks for other games in the series can also be played in this version.